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Nanoscale Biomedical Imaging Facility

The Nanoscale Biomedical Imaging Facility combines state-of-the-art equipment and technical expertise in a user-friendly environment that’s available for scientists, researchers, and collaborators around the world pursuing health-care research through electron microscopy.

 

The facility was founded as a joint facility of Mount Sinai Hospital and The Hospital for Sick Children (SickKids) Research Institute. It now includes infrastructure obtained by SickKids Research Institute, Mount Sinai Hospital, Princess Margaret Cancer Center, and the University of Toronto. It is located in the Peter Gilgan Centre for Research & Learning (PGCRL) at SickKids, and provides microscopy services to scientists throughout Toronto and the scientific community at large.

The Nanoscale Biomedical Imaging Facility provides expertise in electron microscopy, offering imaging solutions for a variety of research endeavours – from molecular and cell biology, to inorganics using cyro-electron microscopy (CryoEM), conventional transmission electron microscopes, (TEM) and 3D-scanning electron microscopes (SEM).


Universities, hospitals, and other institutions across Toronto and Ontario pursuing groundbreaking research need equipment and facilities to match their innovation – that’s what the Nanoscale Biomedical Imaging Facility offers your research team.

The facility consists of three sub-cores:

Cellular and Molecular Electron Microscopy (CMEM)

High-resolution high-throughput CryoEM

3D Scanning Electron Microscopy (3D SEM)

Transmission Electron Microscopy Instruments

Titan Krios G3i, 300 kV Cryo-STEM

  • Falcon 4i camera

  • SelectrisX energy filter

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Glacios 2 200 kV Cryo-STEM

  • Falcon 4i camera

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Scanning Electron Microscopy Instruments

Zeiss Crossbeam 550 SEM

  • 30keV field emission gun

  • Gallium ion beam

  • Carbon and Platinum GIS

  • 54 degree stage tilt

  • Detectors: SESI, Back scattered, eSB, and inLens

  • Isotropic 3D voxel resolution 5nm. Spatial pixel resolution 0.9nm at 15kV

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Zeiss Gemini 450 SEM

  • 30keV field emission gun

  • Detectors: SE, Back scattered, eSB, and inLens

  • Accepts 4” wide wafers

  • Spatial pixel resolution 0.7nm at 15kV

  • Gatan 3View SBF stage with Gatan onPoint back scattered Detector

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